Table 2.
Effects of antiresistin RNA oligo on body weight, metabolic parameters, and hepatic enzymes.
| Paradigm parameter | Chow | HF-Con-oligo | HF-Res-oligo |
|---|---|---|---|
| After treatment Bwt (g) | 28.5 ± 0.9 | 32.8 ± 1.2a | 32.1 ± 1.7d |
| Epididymal fat (g/100 g Bwt) | 1.58 ± 0.05 | 3.3 ± 0.12b | 2.62 ± 0.32ad |
| Serum glucose (mM) | 6.70 ± 0.75 | 9.59 ± 0.72a | 7.22 ± 0.30d |
| Serum insulin (pg/mL) | 528 ± 150 | 689 ± 121a | 503 ± 164d |
| HOMA-IR | 22.8 ± 1.9 | 50.1 ± 6.4b | 27.5 ± 6.2d |
| Serum cholesterol (mM) | 3.90 ± 0.09 | 6.87 ± 0.21b | 4.95 ± 0.37d |
| Serum triglyceride (mM) | 0.63 ± 0.01 | 0.98 ± 0.02b | 0.79 ± 0.04d |
| Serum NEFA (mM) | 0.78 ± 0.16 | 1.03 ± 0.08a | 0.83 ± 0.03d |
| Serum ALT (U/L) | 29.9 ± 0.6 | 89.6 ± 9.5b | 52.1 ± 10.3d |
| Serum AST (U/L) | 32.4 ± 7.9 | 199.8 ± 21.7c | 65.8 ± 11.7f |
| Serum resistin (pg/mL) | 513 ± 36 | 668 ± 35b | 472 ± 19e |
Mice were fed standard chow and high-fat diet for 10 weeks and then with 4-week i.p injection (3 times/week) of saline (Chow, 5 mL/kg of body weight), scrambled control oligo (HF-Con-oligo 25 mg/kg of body weight), or antiresistin RNA oligo (HF-Res-oligo, 25 mg/kg of body weight). Metabolic parameters are represented as mean ± SEM (n = 10 each group) after 4 weeks' treatment. Bwt: body weight; HOMA-IR: homeostatic model assessment-insulin resistance; TG: triglycerides; NEFAs: nonesterified fatty acids; AST: aspartate aminotransferase; ALT: alanine aminotransferase. a P < 0.05, b P < 0.01, and c P < 0.001 HF-Con-oligo group versus chow fed mice; d P < 0.05, e P < 0.01, and f P < 0.001 versus HF-Con-oligo mice.