Table 2.
Box-Behnken design with actual/coded values and results of tetracycline antibiotics recovery.
| X 1 pH | X 2 volume (mL) | X 3 temperature (°C) | Y recovery (%) | |
|---|---|---|---|---|
| 1 | 6 (−1) | 30 (−1) | 30 (0) | 72.20 |
| 2 | 8 (+1) | 30 (−1) | 30 (0) | 74.49 |
| 3 | 6 (−1) | 50 (+1) | 30 (0) | 78.65 |
| 4 | 8 (+1) | 50 (+1) | 30 (0) | 83.15 |
| 5 | 6 (−1) | 40 (0) | 20 (−1) | 77.22 |
| 6 | 8 (+1) | 40 (0) | 20 (−1) | 82.05 |
| 7 | 6 (−1) | 40 (0) | 40 (+1) | 88.63 |
| 8 | 8 (+1) | 40 (0) | 40 (+1) | 88.23 |
| 9 | 7 (0) | 30 (−1) | 20 (−1) | 76.69 |
| 10 | 7 (0) | 50 (+1) | 20 (−1) | 85.70 |
| 11 | 7 (0) | 30 (−1) | 40 (+1) | 83.48 |
| 12 | 7 (0) | 50 (+1) | 40 (+1) | 92.26 |
| 13 | 7 (0) | 40 (0) | 30 (0) | 80.59 |
| 14 | 7 (0) | 40 (0) | 30 (0) | 81.70 |
| 15 | 7 (0) | 40 (0) | 30 (0) | 80.58 |