Figure 3. The TPS10 product TAB can be detected in the open headspace around lox2/3 plants in mixed cultures containing a lox2/3xTPS10 plant.
(A) An ‘open headspace’ trapping was conducted during season two (May 16th) with rosette-stage individuals and edge plants as shown, using activated charcoal filters shielded from ozone and UV by MnO2-coated copper ozone scrubbers (black strips in Teflon tubes pointed at plant) and aluminum foil, respectively. The headspace was sampled during the day, when sesquiterpenes are most abundant (see Appendix 2). Eluents from all four filters were combined and analyzed using highly sensitive GCxGC-ToF analysis; n = 4 plants. (B) The TPS10 product trans-α-bergamotene (TAB) could be detected in the open headspace of two of four lox2/3 plants at the edges of mixed cultures containing a lox2/3xTPS10 plant, but not in lox2/3 individuals or lox2/3 plants in monocultures. Peak areas were normalized to the internal standard (IS) peak. Arrows indicate the plant that was sampled.