Figure 4. Manduca sexta larvae grow larger on LOX2/3-deficient plants, regardless of TPS10 expression, in a glasshouse experiment.
Data are shown as mean ± SEM; n = 15–19 larvae on day 8 and 11–16 larvae on day 12. One M. sexta neonate per plant (starting n = 25 larvae) was placed immediately after hatching on the youngest rosette leaf of an elongated plant. Larvae were weighed at the third and fourth instars (of 5 total), corresponding to days 8 and 12, after which larvae become mobile between plants. a,b/A,B Different letters indicate significant differences (corrected p<0.001) in larval mass on different plant genotypes within each day, in Wilcoxon rank sum tests following significant (corrected p<0.001) Kruskal–Wallis tests for each day (WT vs lox2/3 day 8, W17,19 = 310, corrected p<0.001, day 12, W15,15 = 217, corrected p<0.001; TPS10 vs lox2/3xTPS10, day 8, W15,20 = 290, corrected p<0.001, day 12, W11,14 = 143, corrected p<0.001; WT vs TPS10 day 8, W17,15 = 95, corrected p=0.227, day 12, W15,11 = 56, corrected p=0.361; lox2/3 vs lox2/3xTPS10 day 8, W19,20 = 249, corrected p=0.201, day 12, W16,14 = 134, corrected p=0.377). p-values were corrected for multiple testing using the Holm-Bonferroni method.