Appendix 2—Table 1.
GLVs (percent IS plant-1) | TPS10 products (percent IS plant-1) | Non-target volatiles (percent IS plant-1) | |||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
(Z)-Hexen-3-ol | TAB | TBF | α-Duprezianene | Germacrene A | |||||||||||||||||||||||||||||||||
Genotype | Day n | Night n | Day | Night | Day | Night | Day | Night | Day | Night | Day | Night | |||||||||||||||||||||||||
WT | 8 | 8 | 0.99% | ± | 0.99% | 7.96% | ± | 4.25% | } | alox | 0.37% | ± | 0.29% | a | — | } | aTPS | — | a | — | 2.13% | ± | 0.85% | 0.96% | ± | 0.42% | 1.68% | ± | 0.98% | — | |||||||
TPS10 | 7 | 7 | 2.37% | ± | 1.55% | 2.84% | ± | 0.63% | 18.97% | ± | 6.03% | b | 0.94% | ± | 0.53% | } | 9.34% | ± | 3.44% | b | 0.20% | ± | 0.20% | 9.47% | ± | 5.26% | 0.94% | ± | 0.26% | 2.78% | ± | 1.52% | — | ||||
lox2/3 | 7 | 8 | 0.13% | ± | 0.13% | 1.06% | ± | 0.64% | } | blox | — | a | 0.15% | ± | 0.15% | bTPS | — | a | — | 2.75% | ± | 1.12% | 0.60% | ± | 0.19% | 1.68% | ± | 1.49% | — | ||||||||
lox2/3xTPS10 | 7 | 7 | 0.07% | ± | 0.07% | 1.24% | ± | 0.84% | 7.39% | ± | 2.56% | b | 2.08% | ± | 0.84% | 4.47% | ± | 1.70% | b | 0.40% | ± | 0.40% | 3.02% | ± | 1.42% | 0.73% | ± | 0.31% | 0.66% | ± | 0.37% | — |
a, b Different letters indicate significant differences between lines for a vertical category (corrected P<0.05) in Wilcoxon rank sum tests following significant Kruskal-Wallis tests across all genotypes (see Results text); P-values were corrected for multiple testing using the Holm-Bonferroni method. Community type was also tested and found not significant. Where there are no letters, there are no significant pairwise differences within a category.
}alox, }blox / }aTPS, }bTPS Indicate significant differences (corrected P<0.05) between lines with and without either the lox construct (WT and TPS v. lox and loxTPS) or the TPS construct (WT and lox v. TPS and loxTPS) in Wilcoxon rank sum tests for a category; P-values were corrected for multiple testing using the Holm-Bonferroni method. There are no significant pairwise differences in these categories.
IS, internal standard; GLVs, green leaf volatiles; TAB, (E)-α-bergamotene; TBF, (E)-β-farnesene; —, not detected.