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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Biochemistry. 2008 Mar 13;47(14):4228–4236. doi: 10.1021/bi800023a

Table 2.

Effect of Mutagenesis on the Specificity of saFabI for NADH and NADPH

Enzyme kcat/Km (min−1 μM−1)
NADPH NADH
WT 1.840 ± 0.311 0.010 ± 0.001
R40Q 0.020 ± 0.001 0.053 ± 0.003
K41N 0.036 ± 0.002 0.072 ± 0.002
R40Q/K41N 0.004 ± 0.001 0.076 ± 0.005