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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Mar 14;49(7):2848–2855. doi: 10.1167/iovs.07-1352

Figure 3. Resolution of mitochondrial fraction proteins by 2D gel electrophoresis.

Figure 3

Representative gel demonstrating the resolution of 100 μg of human donor RPE mitochondrial fraction protein. Proteins were separated in the first dimension using a nonlinear pH gradient from 3 to 11 and in the second dimension using 13% SDS-polyacrylamide gel electrophoresis, then stained with silver. Spots that changed significantly by one-way ANOVA or linear regression analysis are boxed and numbered according to Table 2.