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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Mar 14;49(7):2848–2855. doi: 10.1167/iovs.07-1352

Figure 5. 2D gel analysis of mitochondrial ATP synthase content.

Figure 5

A) Mass spectrometry analysis identified multiple ATP synthase α and β protein spots with altered isoelectric focusing points (isoelectric variants, arrows). Only a subset of the isoelectric variants changed significantly with MGS stage (numbered according to Table 2), based upon the initial 2D gel analysis. While the spots identified as ATP synthase α and β migrated as one of several ATP synthase isoelectric variants, ATP synthase δ appeared to migrate as a single spot. B) Total mitochondrial ATP synthase α, β, and δ content was estimated by summing the density of all identified spots (indicated by arrows in A). Mean density values and standard error of the mean were normalized to the greatest density value (MGS 2 for each subunit). The one-way ANOVA for each of the three ATP synthase subunits was significant (all p < 0.04).