Figure 6.

MRS5698 increases phosphorylation of Ser⁹⁴⁰ on KCC2. Compared with contralateral side on D7, MRS5698 (1.0 mg/kg; i.p.), but not vehicle, enhanced pKCC2 immunolabeling (red) in the ipsilateral laminae I and II of the dorsal horn (A; white border) (B, C). Higher-magnification images (yellow box) of superficial dorsal horn are merged with DAPI (blue) (C). Negative controls exhibited low levels of background fluorescence (data not shown). MFI is reported as mean fold change comparing ipsilateral/contralateral. Micrographs represent n = 4 rats/group and 3 sections/animal (L4, L5, and L6 spinal cord levels). Data are mean ± SEM in arbitrary units. *p < 0.05 versus CCI D7 (Welch's corrected one-tailed unpaired Student's t test). Scale bars: A, 200 μm; C, 20 μm.