Figure 10.

CRB1 immunolocalization in BN-H and BN-J retinas. In the BN-H retina, CRB1 localizes in the subapical region above the level of outer limiting membrane (OLM) labeled with GS (A–C, open arrows in the left inset). Higher magnification shows colocalization of CRB1 with GS in the microvilli of RMG cells (arrowheads in the right inset). CRB1 stains also the inner segments (IS) of photoreceptors (A, D). Double staining with PNA (E) shows colocalization of CRB1 with cone IS (F, arrowheads in the inset). In the BN-J retina, CRB1 loses its localization in the subapical region (G–I, inset), OLM is even disrupted in disorganized areas (H, filled arrow). In organized areas, CRB1 remains in the photoreceptor IS (J). Double staining with PNA (K) shows colocalization with cone IS (L, arrowhead in the inset). Scale bar, 20 μm.