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Loss of Müller glial cells in BN-J rat retinas. RMG cells were immunostained with p27kip1, an RMG nuclear marker. P27kip1-positive nuclei in the INL are reduced in the BN-J rat retina (C) compared with BN-H rat retina (A). B and D are merged images with DAPI. Scale bar, 50 μm. Cell count of p27kip1-positive RMG shows significant decrease in BN-J rat retinas (E). n = 7 rats for BN-H and 6 rats for BN-J; **p < 0.01.
