Figure 3.

EPSC properties differ between pyramidal cell lineages. A, AMPA-R mediated sEPSCs recorded from representative non-Tbr2 (green) and Tbr2 (red) lineage neurons demonstrating similar frequencies of events in the two groups. B, Averaged waveforms of several hundred sEPSCs from typical non-Tbr2 and Tbr2 lineage neurons. The waveform obtained from the non-Tbr2 lineage neuron was overlaid on that from the Tbr2 lineage neurons (overlay) and then normalized to demonstrate that the difference in decay time and area is linked to the difference in amplitude. C, Bar graphs demonstrating significant differences in the amplitude, decay time, and area, but not frequency, of sEPSCs in neurons from the two lineages. *p < 0.05. D, E, Scatter plots demonstrating no significant relationship between Rn and sEPSC amplitude (R² values, Tbr2 lineage = 0.000028, non-Tbr2 lineage = 0.166) or area (R² values, Tbr2 lineage = 0.001, non-Tbr2 lineage = 0.000044). Calibration: A, 20 pA/50 ms; B, 4 pA/5 ms.