Figure 4.

Precursor lineages generate pyramidal neurons with different morphology. A, Representative confocal images showing that Tbr2 (mCherry) and non-Tbr2 (ZsGreen) neurons can be distinguished following biocytin staining (blue). Biocytin-filled fate mapped neurons were scanned at high resolution using confocal microscopy, and their entire dendritic arbor was reconstructed. B, C, Representative neuron reconstructions from each lineage arranged based on depth within layers 2 and 3 demonstrating greater dendritic complexity in the non-Tbr2 lineage. D, Sholl analysis demonstrates increased apical dendrite complexity in non-Tbr2 lineage neurons compared with the Tbr2 lineage, whereas no significant differences were found in the basal dendrites (E). *p < 0.05. F, G, The number of apical branches and total dendrite length were increased in non-Tbr2 lineage neurons. **p < 0.01. H–J, Convex hull analyses demonstrate no difference in the volumes of the apical or basal compartments (I) or in dendrite length per unit volume (J).