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Experimental timeline: Rats are exposed to alcohol vapor for 7 weeks (14 h per day). A, Amg, Hipp, NAc, and mPFC were collected 3 weeks after the end of alcohol exposure. B, Alcohol self-administration (SA) was measured 3 weeks after alcohol exposure. Once SA was stable (baseline), cannulae connected to an osmotic mini-pump containing either RG108 or vehicle were implanted into mPFC of PD and control rats. The rats were tested for SA after 1 week recovery.
