Figure 8.

Syt2 inhibition increases tolerance to quinine adulteration. A, Immunohistochemical detection of syt2 (red) and cell infected by shRNA lentiviral vector specific to syt2. The figure shows that cells infected by lentivirus do not express SYT2 (green). B, Number of reward for alcohol in rats that received injection of shRNA lentiviral vector specific to Syt2 (square) and rats that received the scrambled lentiviral vector. C, Compulsive-like drinking (i.e., persistent alcohol drinking despite the aversive bitter taste of quinine added to the alcohol solution). The data represent the percentage change from baseline (i.e., lever presses for alcohol alone before adulteration with quinine). *p < 0.05; scramble vs. Syt2. D, Syt2 inhibition does not modify quinine consumption. Bar graph represents quinine consumption as measured by two-bottle free choice. E, Graph shows mapping of viral injection sites within the mPFC (•).