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. 2015 Apr 15;5:9675. doi: 10.1038/srep09675

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(a) 293T-CD1d cells were transfected with the indicated non-overlapping triple-alanine mutants of the WITO Vpu C-terminus. 24 h post transfection, cells were surface stained with anti-CD1d antibody and analyzed by flow cytometry. Average values from at least 3 independent experiments preformed in duplicates (±SD) are shown. Statistical analysis was done using GraphPad Prism software and one-way ANOVA with Dunnett's multiple comparisons test. (b) Vpu amino acid sequences of subtype B (n > 5500) and subtype C (n > 3000) were retrieved from the HIV sequence database (www.hiv.lanl.gov) and the C-terminal 22 residues of the sequences analyzed using WebLogo 3.0 software (weblogo.threeplusone.com/create.cgi). Colour coding of the amino acids represents hydrophobicity; blue, hydrophilic; green, neutral; black, hydrophobic. Conserved motifs are highlighted.