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. Author manuscript; available in PMC: 2015 Sep 16.
Published in final edited form as: Nat Commun. 2015 Mar 16;6:6380. doi: 10.1038/ncomms7380

Figure 6. Epsin 1 interacted with Dvl2 via its ENTH and UIM domains.

Figure 6

Epsin 1 full length (Epsin 1) and the following truncated epsin 1 constructs were co-transfected with full length Dvl2 into HEK 293T cells: HA-tagged full length epsin 1 (Epsin 1) (a,b,d), Epsin 1 ΔUIM (ΔUIM) (a), Epsin 1 ΔDPW (ΔDPW) (a), ENTH alone (ENTH) (a), NPF alone (NPF) (a), UIM alone (UIM) (b) and ENTH-UIM alone (ENTH-UIM) (d); Flag-tagged Epsin 1 full length (Epsin 1) (c), Epsin 1 ΔENTH (ΔENTH) (c) and DPW-NPF alone (DPW-NPF) (c). (a–d) Dvl2 was co-immunoprecipitated by specified epsin 1 constructs using anti-HA (a,b,d) or anti-epsin 1 (c) antibodies, then analyzed by Western blotting. All are representative blots from n=8.