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. 2015 Apr 10;13:27. doi: 10.1186/s12958-015-0023-2

Figure 1.

Figure 1

Dose-dependent productions of IL-8 and GROα stimulated with TNF- α . Dose-dependent productions of IL-8 (A) and GROα (B) in KGN cells stimulated with TNF-α. Cultured cells were incubated with the indicated concentrations of TNF-α for 24 h at 37°C in 5% CO2. At the end of the incubation period, the conditioned medium was collected and assayed for IL-8 and GROα concentrations by ELISA. The values represent the relative ratios of IL-8 and GROα concentrations, compared with those in untreated cells after normalization with total protein. Values are the mean ± SD of the combined data of four separate experiments using different KGN preparations. A: *P < 0.01, **P < 0.001 (all vs.control). B: *P < 0.01; ** P < 0.001 (all vs. control).