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Significant higher expression level of stemness markers (NANOG, OCT-4, SOX-2 and REX-1) were observed in cryopreserved ASCs (*p<0.05 relative to fresh ASCs). Cryoprotective agent: 1) 0.25 M trehalose; 2) 5% dimethylsulfoxide (DMSO); 3) 10% DMSO; 4) 5% DMSO + 20% fetal bovine serum (FBS); 5) 10% DMSO + 20% FBS; 6) 10% DMSO + 90% FBS.
