Table 1.
Inhibition as observed after EZ1-based nucleic acid extraction and after column-based nucleic acid extraction for various applied PCR test kits. The in-house malaria PCR did not include an internal inhibition-control approach
| Applied PCR kit | Number of analyzed samples (n) | Inhibition after EZ1 extraction only (n) | Inhibition after column-based extraction only (n) | Inhibition after both extraction schemes (n) |
|---|---|---|---|---|
| Malaria PCR Kit 1.0 (altona DIAGNOSTICS) | 48 | 4 | 0 | 0 |
| In-house real-time multiplex PCR for enteroinvasive bacteria according to [30, 31] | 81 | 7 | 0 | 1 |
| ETEC-EIEC kit (RidaGene) | 81 | 0 | 0 | 1 |
| EHEC-EPEC kit (RidaGene) | 81 | 0 | 0 | 1 |
| EAEC kit (RidaGene) | 81 | 1 | 0 | 1 |
| In-house real-time multiplex PCR for enteropathogenic protozoa according to [32–34] | 81 | 10 | 0 | 2 |
| Viral gastroenteritis kit (Fast-track Diagnostics) | 81 | 0 | 0 | 0 |