Figure 4.

Suppressive activity of murine CD4⁺ Tregs and CD8⁺ Tregs. Freshly sorted CD4⁺GFP⁻ (CD4⁺ Teffs) cells were cultured with CD4⁺ Tregs or CD8⁺ Tregs at various ratios for 3 days, and tritiated thymidine was added for the last 16 h of the culture. Proliferation was assessed by tritiated thymidine incorporation measurement.