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. 2015 Mar 29;12:49. doi: 10.1186/s12985-015-0275-7

Figure 1.

Figure 1

Specificity Characterization of the polyclonal antibody against BP53 by western blot. Line marker, pre-stained protein molecular mass markers (MBI, USA); Line 1 to Line 3, SDS-PAGE of gill membrane proteins extracted from WSSV-free Litopenaeus vannamei (Line 1), the lysates from E. coli Top10 cells with blank plasmid pBAD-gIIIB (Line 2, negative control), lysates of E. coli Top10 cells with recombinant plasmid pBAD-gIIIB-BP53 (Line 3). Line 4 to Line 6, identification of BP53 using anti-rBP53 antibody by western blot. Samples loaded were as same as Line 1 to Line 3 in sequence.