Video 10. Some filopodia adhere to somatic cells and appear to be under tension.
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DOI: 10.7554/eLife.05279.038
Laser-induced cut of a filopodium was performed using a Zeiss LSM710 confocal microscope equipped with a Zeiss W Plan-Apochromat 63× objective controlled by the ZEN software (Zeiss, Germany). Following cut, the tip of the filopodium remained in contact with the distant somatic cell, while the part of the protrusion attached to the cell rapidly retracted, signifying tension. The video spans 7 s with snapshots captured at a 200 ms time-interval (pinhole 208 µm, 128 × 128). Time indicated in seconds and scale bar represents 5 µm.