Video 2. Filopodia are not directly controlling bleb formation.
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DOI: 10.7554/eLife.05279.011
Bleb-formation (asterisk) can be observed first in close proximity to filopodia (arrowheads, 10–20″) and then in a region devoid of filopodia (40″). A 40-s time-lapse video of a membrane-labelled PGC (in a kop-egfp-f'nos3′UTR embryo) using a 63× objective on a Zeiss AxioImager.M2 microscope equipped with a dual view filter (MAG Biosystems), Photometrics camera (Cascade II) and VS-Laser Control. Z-stacks were captured with 29 planes at focal planes per time point, 1 µm apart at 10 s interval, 300 ms exposure time and with binning one. Scale bar represents 10 µm.