Video 4. Low concentration of uniformly expressed Cxcl12a affects cell migration, but does not immobilize the cells.
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DOI: 10.7554/eLife.05279.016
Migration tracks of PGCs in medNY054 homozygous embryos knocked down for Cxcr7b, injected with mcherry_h2b_globin3′UTR mRNA (mCherry labelling nuclei of all cells) and either with 2 pg control mRNA (first video) or 2 pg cxcl12a mRNA (second video). Tracks represent 58 min of development in 7 hpf embryos. Snapshots captured at 2-min intervals with 300 ms exposure time at three focal planes (15 µm apart) using a 10× objective to generate the Z-stacks on a Zeiss AxioImager.M1 microscope equipped with a Photometrics camera (CoolSNAP ES2). Time in minutes and scale bar represents 50 µm.