Video 8. Cxcl12a internalization.
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DOI: 10.7554/eLife.05279.025
Cxcl12a (green) is bound to the PGC membrane (magenta) and internalizes into the cell. The video was captured using a 63× objective on a Zeiss AxioImager.M2 microscope equipped with a Photometrics camera (Cascade II) and VS-Laser Control at 15 s intervals for 2:15 min and shows an optical section of a PGC (a Z-projection of two 1-µm-slices). Arrows indicate a bound and an internalizing Cxcl12a spot. Scale bar is 5 µm.