Skip to main content
. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Free Radic Biol Med. 2013 Jul 15;65:371–379. doi: 10.1016/j.freeradbiomed.2013.07.009

Fig. 4.

Fig. 4

miR-761 can inhibit mitochondrial fission and apoptosis. (A) miR-761 is downregulated upon hydrogen peroxide treatment and addition of NAC abolished downregulation of miR-761 induced by hydrogen peroxide. Cardiomyocytes were pretreated with 10 mM NAC and then treated with 100 μM hydrogen peroxide. miR-761 levels were detected by qRT-PCR. (B and C) miR-761 suppresses hydrogen peroxide-induced mitochondrial fission and apoptosis. Cardiomyocytes were transfected with miR-761 mimic or NC. After that, they were treated with 100 μM hydrogen peroxide. (B) Mitochondrial fission and (C) apoptosis were assayed. *P<0.05 versus hydrogen peroxide alone. (D) Analysis of the expression levels of miR-761. Mice were subjected to various periods of ischemia and the expression of miR-761 was assayed by qRT-PCR. *P<0.05 versus sham or 0 min group. (E and F) miR-761 attenuates mitochondrial fission and apoptosis upon I/R injury. Adult C57BL/6 mice received miR-761 mimic or mimic-NC for 3 days. They were then subjected to I/R. (E) The percentage of mitochondrial fission was calculated. (F) Apoptosis was detected by TUNEL. *P < 0.05 versus I/R alone.