Table 1.
A full toolset of plasmid variants for IUE.
| Plasmid name | ITR-flanked cassette | Subcellular target | Addgene # |
|---|---|---|---|
| pPBC-LG6f | Lck-GCaMP6f | Plasma membrane | 62808 |
| pPBC-LG6s | Lck-GCaMP6s | Plasma membrane | 62809 |
| pPBC-LG6f-tdT | Lck-GCaMP6f-IRES-tdTomato | Plasma membrane | 62807 |
| pPBC-LG3-tdT | Lck-GCaMP3-IRES-tdTomato | Plasma membrane | 62810 |
| pPBC-G6f | GCaMP6f | Cytosol | 62811 |
| pPBC-G6s | GCaMP6s | Cytosol | 62812 |
| pPBC-G6f-tdT | GCaMP6f-IRES-tdTomato | Cytosol | 62813 |
| pPBC-G3-tdT | GCaMP3-IRES-tdTomato | Cytosol | 62814 |
All piggyBac ITR-flanked cassettes are driven by the CAG promoter. Plasmids are co-electroporated with a piggyBac transposase-expressing plasmid (Wu et al., 2007).