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(a) Distribution of unbinding forces between anti-TfsA antibody and the bacterial cell surface (wild-type and S-layer single mutants) at a retraction velocity of 1000 nm/s, (b) comparison of binding probabilities of the anti-TfsA antibody-conjugated AFM tip to wild-type, TfsB-deficient (ΔtfsB) and TfsA-deficient (ΔtfsA) cells, as well as the blocking experiment, and (c) a plot of loading rate dependence versus unbinding force.
