Table 1.
Oligonucleotide Sequences
| Oligonucleotides | Sequence |
|---|---|
| Biotin-T45U5* | 5′-Biotin-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT UUUUU-3′ |
| Adapter A** | 5′-/5rApp/NNNNGATCGTCGGACTGTAGAACTCTGAAC/3ddC/-3′ |
| Primer B | 5′-GTTCAGAGTTCTACAGTCCGACGATC-3′ |
| Adapter C | 5′-CCUUGGCACCCGAGAAUUCCANNNN-3′ |
| lniversal primer | 5′-AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA-3′ |
| Index primer 1 (ATCACG) | 5′-CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 2 (CGATGT) | 5′-CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 3 (TTAGGC) | 5′-CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 4 (TGACCA) | 5′-CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 5 (ACAGTG) | 5′-CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 6 (GCCAAT) | 5′-CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 7 (CAGATC) | 5′-CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 8 (ACTTGA) | 5′-CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 9 (GATCAG) | 5′-CAAGCAGAAGACGGCATACGAGATCTGATCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 10 (TAGCTT) | 5′-CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 11 (GGCTAC) | 5′-CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Index primer 12 (CTTGTA) | 5′-CAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
| Sequencing primer | 5′-CTACACGTTCAGAGTTCTACAGTCCGACGATC-3′ |
| Index sequencing primer | 5′-GGAATTCTCGGGTGCCAAGGAACTCCAGTCAC-3′ |
| Q-PCR checking primer F | 5′-CAAGCAGAAGACGGCATACGA-3′ |
| Q-PCR checking primer R | 5′-AATGATACGGCGACCACCGAGAT-3′ |
Italic letter represents an RNA nucleotide; N represents a random nucleotide.
Bold letters indicate an index region. Please note that the index sequence on the read is the reverse complement of the index sequence (in parentheses).
*
Biotin-T45U5 is a chimeric DNA/RNA oligonucleotide with a standard biotin modification at the 5′ end.
**
/5rApp/ refers to 5′ adenylation and /3ddC/ refers to dideoxycytidine, a 3′ chain terminator.