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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: J Biol Chem. 1999 Jul 30;274(31):22025–22032. doi: 10.1074/jbc.274.31.22025

Fig. 9. Competition of Fl-PNA/pDNA nuclear import by plasmid DNA and BSA-NLS.

Fig. 9

Permeabilized HeLa cells were incubated for 4 h at 37 °C with transport buffer containing HeLa cytoplasmic extract (5 mg/ml) and Fl-PNA/pUSAG3 (10 µg/ml) in the absence (A) or presence of a 1000-fold molar excess of BSA-NLS (0.23 mg/ml; B), a 100-fold excess of pUC19 (0.7 mg/ml; C), or a 100-fold molar excess of SV40 DNA (1.3 mg/ml; D).