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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Mol Cell. 2014 Sep 4;55(6):829–842. doi: 10.1016/j.molcel.2014.08.002

Figure 6. LIG3 deficiency does not affect translocations in human cells.

Figure 6

A. – C. Neither translocation frequency nor junction characteristics are altered by LIG3 loss. Translocations are induced by expression of the indicated nucleases: ZFN, ZFNp84+ZFNEWS, generating Der22 (n = 3); Cas9, Cas9+gRNAs (ALK1+NPM1) or nCas9, nCas9+gRNAs (ALK1+ALK2 and NPM1+NPM2), generating Der5 (n = 2). B, Translocation frequency. Error bars, +/− SEM. C, Deletion lengths from individual Der22 (ZFNs) and Der5 (Cas9, nCas9) junctions. D, Microhomology distribution. Microhomology in junctions from control and LIG3-deficient cells was similar.

See also Figure S6.