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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Methods Enzymol. 2014;546:251–271. doi: 10.1016/B978-0-12-801185-0.00012-X

Figure 3. Cas9 cleavage at the NPM and ALK loci and example of a translocation junction sequence.

Figure 3

A. Cas9 cleavage at the NPM and ALK loci using the NPM1 and ALK1 gRNAs, respectively, leads to DSBs. Only the part of the gRNA that binds DNA is shown (black). PAM sequence, boxed; arrows, cleavage sites.

B. DNA ends from Cas9 cleavage that are relevant to Der5 formation are shown. NHEJ leads to a variety of Der5 translocation junctions, but a common junction (shown) is direct ligation of the DNA ends, presumably involving fill-in of the 1-base 5′ overhang.