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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Methods Enzymol. 2014;546:251–271. doi: 10.1016/B978-0-12-801185-0.00012-X
NPM-ALK
Cas9-generated DSBs with gRNAs NPM1 and ALK1; nCas9-generated paired nicks with gRNAs NPM1+NPM2 and ALK1+ALK2 (Fig. 1B).
NPM genomic sequence followed by gRNA target sequences:
  • 5′-CCTCGAACTGCTACTGGGTTCACCTCAGCCTCTGGAATAGCTAGAACTACAGG-3′
  • gRNA NPM1: 5′-GTGAACCCAGTAGCAGTTCG-3′
  • gRNA NPM2: 5′-GCCTCTGGAATAGCTAGAACTAC-3′
ALK genomic sequence followed by gRNA sequences:
  • 5′-CCTCAGGTAACCCTAATCTGATCACGGTCGGTCCATTGCATAGAGGAGG-3′
  • gRNA ALK1: 5′-GATCAGATTAGGGTTACCTG-3′
  • gRNA ALK2: 5′-GTCGGTCCATTGCATAGAGG-3′