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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Methods Enzymol. 2014;546:251–271. doi: 10.1016/B978-0-12-801185-0.00012-X
EWS-FLI1
EWS genomic sequence followed by gRNA sequence:

  • 5′-GGAATCCAGGACACATCTTTAGG-3′

  • gRNA EWS: 5′-GGAATCCAGGACACATCTTT

Potential off-target site for the gRNA EWS on Chr9 (2 mismatches) (Fig. 2A):

  • 5′-GGAT*TCCAGGACC*CATCTTTGGG-3′

FLI1 genomic sequence followed by gRNA sequence:

  • 5′-CCTCCCATGGCTGTCTCTAGACC-3′

  • gRNA FLI1: 5′-GGTCTAGAGACAGCCATGGG