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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Methods Enzymol. 2014;546:251–271. doi: 10.1016/B978-0-12-801185-0.00012-X
EWS locus primers generate a 354-bp fragment from the wild-type locus and smaller fragments from the modified locus (Fig. 2B):
  • EWS-F 5′-CCTCAGCCACCCAGAGTGTT-3′
  • EWS-R 5′-TAGCTGCCTCCCCACTTTACAT-3′
EWS off-target locus primers generate a 474-bp fragment from the wild-type locus and smaller fragments from the modified locus
  • EWS-OFF-F 5′-ACTCACCTGGTTGGGTTGTCTT-3′
  • EWS-OFF-R 5′-GTCCGTACTATGAAGGGGTCGT-3′