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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Methods Enzymol. 2014;546:251–271. doi: 10.1016/B978-0-12-801185-0.00012-X
Der22, corresponding to the EWS-FLI1 fusion (Fig. 2B):
External primers:
  • Der22-F 5′-ATCCTACAGCCAAGCTCCAA-3′
  • Der22-R 5′-GGCCTCATTGTTTCTGGCTA-3′
Internal primers:
  • Der22-NF 5′-CTACGGGCAGCAGAGTGAGT-3′
  • Der22-NR 5′-TTCCTCAAGGCTCTGGAAAA-3′
Der9, corresponding to the Off-target-FLI1 fusion (Fig. 2B):
External primers:
  • Der9-F 5′-TAGTGGGGGAAGGAGAGACA-3′
  • Der9-B 5′-GCCAGGTTTCTTAGGGCTTT-3′
Internal primers:
  • Der9-NF 5′-GAAAAGGCTCCATTTCATGC-3′
  • Der9-NB 5′-GGGCTGAGCTCCATAAATCA-3′