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. 2015 Jun;93:28–40. doi: 10.1016/j.neuropharm.2015.01.001

Fig. 5.

Fig. 5

α-Bgtx-AF555 binds to β3 but not β1 or β2 subunits. A, Images of HEK-293 cells expressing eGFP with either β1, β2 or β3 subunits, incubated with 400 nM α-Bgtx-AF555 for 10 min at RT, 48 h after transfection, washed to remove the excess α-Bgtx-AF555, fixed and imaged. B, Mean surface membrane fluorescence of cells expressing eGFP and β1–3 subunits. ***P < 0.001, n = 6–9. C, Images of HEK-293 cells expressing eGFP with or without either β1, β2 or β3 subunits, after fixation in 4% PFA, 48 h after transfection, and permeabilised with 0.1% w/v Triton-X100, and incubated in 400 nM α-Bgtx-AF555 for 10 min at RT, washed and imaged. Arrowheads indicate intracellular structures labelled with α-Bgtx-AF555. Scale bars 5 μm (A) and 10 μm (C).