Fig. 9.

α-Bgtx does not inhibit GABA-activated α4β2δ currents. A, Representative whole-cell GABA-activated currents from HEK-293 cells expressing α4β2δ receptors in response to sub-maximal (1 μM) and maximal GABA concentrations (1 mM) in comparison to 300 μM THIP. B, GABA concentration response curve for α4β2δ receptors expressed in HEK-293 cells. Experiments were performed 48 h after transfection. pEC₅₀: 6.04 ± 0.12 (n = 5) and EC₅₀ = 0.91 μM. C, Representative whole-cell GABA-activated currents in response to submaximal (1 μM) concentrations of GABA in the absence (left-panels) and presence of 5 and 20 μM α-Bgtx (pre-applied for 30s) in HEK-293 cells. D, Potentiation of 1 μM GABA-activated current by 5 μM α-Bgtx compared to control (Con). E, Potentiation of 1 μM GABA-activated current by 20 μM α-Bgtx compared to control (Con). F, Representative whole-cell GABA-activated currents in response to a maximal GABA concentration in the absence (left panel) and presence (right panel) of 5 μM α-BgTx with (bottom panel) or without (top panel) a 30s pre-application of α-BgTx. G, Representative GABA-activated maximal currents before, during, and after application of 5 μM α-Bgtx. Note the change in leak current during α-Bgtx). H, Changes to leak current in control (Con), +5 μM and +20 μM α-Bgtx *P < 0.05, **P < 0.01, ***P < 0.001, n = 3–7, unpaired two-tailed t-test and One-way ANOVA.