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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Sci Transl Med. 2014 Dec 10;6(266):266ra172. doi: 10.1126/scitranslmed.3009644

Fig. 6. LBSC treatment influences light transmission properties of ECM deposited after debridement.

Fig. 6

(A) Macroscopic images of mouse eyes in diffuse lighting reveal opaque scars (arrow) in untreated (no cells) eyes but none in LBSC-treated corneas. (B) OCT imaging shows transverse optical sections of preoperative (Pre-op) eyes and those 2 and 4 weeks after debridement, with scarring visible as bright pixels in the corneal stroma. (C) Thresholding of high-intensity pixels in three-dimensional (3D) OCT images of individual corneas defines scarred region (green) at 2 and 4 weeks after debridement. (D) Light scatter in 3D OCT scans at 2 and 4 weeks was compared with the values in preoperative eyes. Data are means ± SD. The number of eyes is indicated in the graph. P values were determined with unpaired t tests at each time point compared to respective Pre-op values (table S4). (E) Transmission electron micrographs 4 weeks after debridement show the ablated region of the anterior stroma. epi, epithelial cells; bm, basement membrane; k, keratocyte processes; L, amorphous matrix deposit (lake). Insets show magnification of a box (1 μm × 1 μm) from the indicated region containing orthogonal views of collagen fibrils. Scale bar, 2 μm. Images are representative of n = 3 animals.