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. 2015 Apr 15;10(4):e0122101. doi: 10.1371/journal.pone.0122101

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© 2015 Yu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Luciferase assay. (B) Split luciferase to give two complementary fragments N-Luc 2–416 and C-Luc 398–550, each his-tagged at N terminus and C terminus respectively (the structure is from PDB ID 2D1S) [39]. (C) SDS-PAGE picture of N-Luc and C-Luc.