Fig 1. Effects of MK591 on the morphology and viability of C4-2B cells.
C4-2B prostate cancer cells and normal HFF cells (~3 x 105 cells per plate) were plated in 60 mm diameter plates and allowed to grow for 48 hours. Then the cells were treated with varying doses of MK591 for 72 hours at 37°C in the incubator. Ibuprofen (a cyclooxygenase inhibitor) was used as negative control. At the end of incubation period, photographs were taken with a Nikon digital camera attached to a Leica microscope at x400 and processed on a Dell computer using Q-Capture Pro7 software. In (c), comparative effects of MK591 on the viability of cancer (C4-2B) and normal (HFF) cells are shown after treating cells under the same experimental conditions. Control cells were treated with vehicle only (0.2% DMSO). At the end of treatment period, cell viability was measured by MTS/PES assay as described before (20–23). Results are quantitatively presented as mean values of each data point ± standard error (n = 4). ** p = <0.005. In (d and e), Expression of 5-Lox in HFF and C4-2B cells was detected by RT-PCR and Western blot as described in the “Methods” section.