Table 2. Peptidase absolute and specific activities involved in protein digestion in MMG from the scorpion Tityus serrulatus using different substrates.

Substrate; pH	Absolute Activity (U/MMG)	Specific Activity (U/mg)
Z-FR-MCA (3)	580 ± 80	16 ± 2
Z-FR-MCA (5.5)	700 ± 234	15 ± 5
Z-FR-MCA (8)	43 ± 2	1.2 ± 0.2
Z-RR-MCA (5.5)	81 ± 16	1.6 ± 0.2
N-Suc-AAPF-MCA (8)	4 ± 1	0.1 ± 0.04
Casein-FITC (8.5)**	2.1 ± 0.4	0.06 ± 0.03
Hemoglobin (2.8)**	28 ± 4	0.93 ± 0.04
Abz-FRQ-EDDnp (3)	35 ± 3	0.8 ± 0.2
Abz-FRQ-EDDnp (5.5)	2.2 ± 0.7	0.06 ± 0.02
Abz-GIVRAK-EDDnp (3)	0.42 ± 0.06	0.009 ± 0.001
Abz-GIVRAK-EDDnp (5.5)	0.16 ± 0.03	0.005 ± 0.001
Abz-GIVRPK-EDDnp (3)	0.18 ± 0.03	0.004 ± 0.001
Abz-GIVRPK-EDDnp (5.5)	0.2 ± 0.03	0.005 ± 0.001
Abz-GIVRAK-(Dnp)OH (3)	0.77 ± 0.06	0.018 ± 0.003
Abz-G-I-V-R-A-K-(Dnp)OH (5.5)	0.9 ± 0.3	0.025 ± 0.09
Abz-G-I-V-R-P-K-(Dnp)OH (3)	0.270 ± 0.004	0.006 ± 0.001
Abz-G-I-V-R-P-K-(Dnp)OH (5.5)	0.53 ± 0.04	0.015 ± 0.001
Abz-G-P-K-R-A-P-W-V-EDDnp (8)	0.9 ± 0.1	0.02 ± 0.004

Values are means and S.E.M of cleaved substrates in at least three different biological samples from the MMG of Tityus serrulatus. Assay conditions are listed in text.