(A–D) Triple immunofluorescence (IF) targeting cell proliferation (Ki67, blue) in the bulge (Bu) hfSCs using CD34 (red) marker expression and the hair germ (HG) using P-cadherin (green) staining in Con (A, C) and Wnt7b cKO (B, D) HFs at P21 (A–B) and P24 (C–D). (E) Quantification of nuclear Ki67 IF in specific HF compartments (Bu, bulge hfSCs, HG, hair germ; Mx, matrix cells) at P21 and P24. (F–F’) FACS analysis of P18 Con (F) and Wnt7b cKO (F’) HFs employing CD34 and α6-integrin marker expression. α6-integrinHigh/CD34High (red) denotes comparable total basal hfSCs (b-hfSCs) numbers in the bulge of Con (E, 39.8%) and Wnt7b cKO (E’, 40%) HFs.