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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Stem Cells. 2014 Apr;32(4):886–901. doi: 10.1002/stem.1599

Figure 4. Wnt7b-deficiency does not perturb the sequential “two-step” mechanism of HF activation during telogen-anagen transision.

Figure 4

(A–D) Triple immunofluorescence (IF) targeting cell proliferation (Ki67, blue) in the bulge (Bu) hfSCs using CD34 (red) marker expression and the hair germ (HG) using P-cadherin (green) staining in Con (A, C) and Wnt7b cKO (B, D) HFs at P21 (A–B) and P24 (C–D). (E) Quantification of nuclear Ki67 IF in specific HF compartments (Bu, bulge hfSCs, HG, hair germ; Mx, matrix cells) at P21 and P24. (F–F’) FACS analysis of P18 Con (F) and Wnt7b cKO (F’) HFs employing CD34 and α6-integrin marker expression. α6-integrinHigh/CD34High (red) denotes comparable total basal hfSCs (b-hfSCs) numbers in the bulge of Con (E, 39.8%) and Wnt7b cKO (E’, 40%) HFs.