Skip to main content
. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Biochem J. 2011 Aug 15;438(1):39–51. doi: 10.1042/BJ20110129

Figure 6. Effect of antibodies on plasmin-catalysed cleavage of single-chain pro-uPA.

Figure 6

Aliquots of pro-uPA (200 nM) were incubated with plasmin (5 nM) in the absence or presence of mAb-PUK (300 nM). After the indicated incubation periods, the reaction products were analyzed by reducing SDS-PAGE and immunoblotting with a polyclonal anti-uPA antibody. Two-chain active uPA was added to lane one as a control. The cleavage by plasmin was observed as the conversion of the Mr ~54,000 band of single-chain pro-uPA to the Mr~34,000 catalytic domain and Mr~20,000 amino-terminal fragment of two-chain uPA.