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. 2015 Apr 15;10(4):e0122577. doi: 10.1371/journal.pone.0122577

Fig 5. Atorvastatin calcium inhibited the PDGF-BB-induced phenotype switching of VSMCs.

Fig 5

In (A), Con, VSMCs were cultured without any treatment; ATV, VSMCs were treated only with atorvastatin calcium (10μM) for 24h; PDGF-BB, VSMCs were treated only with PDGF-BB (20ng/ml) for 24h; PDGF-BB+ATV, VSMCs were treated with atorvastatin calcium (10μM) and PDGF-BB (20ng/ml) for 24h. The protein expression levels of the smooth muscle markers αSMA, SM22α and calponin were determined by western blotting. GAPDH, glyceraldehyde 3-phosphate dehydrogenase. In (B) (C) (D), the band intensity in (A) was quantified using Image J 1.47 software and relative expression averaged across the three experiments. Probability values are indicated above bars: *p<0.05, **p<0.01, ***p<0.001 versus control.