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. 2015 Apr 15;10(4):e0124216. doi: 10.1371/journal.pone.0124216

Fig 4. Effects of PIA/PNAG on the adherence of S. carnosus TM300 to epithelial cells.

Fig 4

PIA/PNAG production (A), biofilm formation (B) and adherence of S. carnosus TM300 and S. carnosus TM300(pTXicaADBC) to RPMI 2650 cells (C) were determined. (D) EPS that was purified from S. aureus SA113 at various concentrations was mixed with S. carnosus TM300 and incubated with RPMI 2650 cells. The average number of S. carnosus TM300 that adhered to each RPMI2650 cell was determined. EPS (2.4 μg/ml) from S. aureus SA113, SA113Δica, S. epidermidis O-47 and S. epidermidis O-47ΔicaB was treated or untreated with 0.5 mg/ml dispersin B. The amounts of PIA/PNAG were determined by WGA-biotin (E). The average number of S. carnosus TM300 adhered to each RPMI2650 cell was determined by CFU enumeration (F). Significant differences are denoted with ***p-value < 0.001.