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In vitro metacyclogenesis using TAU medium of lines harboring transgenes encoding TcSIR2RP1HA and TcSIR2RP3HA uninduced (- Tet) or induced (+ Tet) with 0.5 μg/ml Tetracycline. The bar graph represents the mean ± SEM from three independent experiments; **** p<0.0001 (unpaired, two-tailed Student t test).
