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. 2015 Apr 15;10(4):e0119960. doi: 10.1371/journal.pone.0119960

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© 2015 Obana-Koshino et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — E-cadherin mRNA expression levels were analyzed. E-cadherin mRNA expression levels in SMG cultured with 1 μM and 100 μM melatonin were normalized to that in control SMG without melatonin (n = 3) (A). The expression of E-cadherin protein was analyzed by Western blot. The study was repeated three times, with data shown from a representative experiment (B). The band intensity was quantitated (n = 3) (C). The adherence junction is shown in the presence of melatonin by TEM (D, E, F). The localization of E-cadherin was shown in the presence of melatonin by immuno-electron microscopy (G, H, I). Scale bar: 500 nm. Bars represent the mean ± SEM. **p < 0.01 compared with control.