Table 2.

Primers for site-directed mutagenesis and RT amplification

Amino acid substitution/name	Genetic mutation	Sequence (5′–3′)
Primers for site-directed mutagenesis
Y181C_F	A3062G	TATCTGTCAATACATGGATGACTTGTATGTAGGATCTG
Y181C_R	A3062G	TATTGACAGATAACTATGTCTGGATTTTGTTTTC
H221Y_F	C3181T	AAGAAATATCAGAAAGAACCTCCATTTCTTTGGATGGGGTATG
H221Y_R	C3181T	TTCTGATATTTCTTGTCTGGTGTGGTAAATCCCCACTTTAACAG
D404N_F	G3730A	TGGACAAACTGGTGGCAAGCCACCTGGATTC
D404N_R	G3730A	ACCAGTTTGTCCACCATGTCTCCCATGTTTCTT
D404E_F	C3732G	TGGACAGAGTGGTGGCAAGCCACCTGGATTC
D404E_R	C3732G	ACCACTCTGTCCACCATGTCTCCCATGTTTCTT
Primers for RT amplification
HI1395		ATGAAGAGGCTGCAGAATGGG
HI4635		GGATTCTACTACTCCCTGACTTTGG

Mutated nucleic acids in the primer sequences are shown in bold.